Technician – In Vivo Models/Immunotherapy/Genetic Engineering
Kennziffer: 2025-0114
- Heidelberg
- Full-time
- Pediatric Immuno-Oncology
„Forschen für ein Leben ohne Krebs“ – das ist unsere Aufgabe am Deutschen Krebsforschungszentrum. Wir erforschen, wie Krebs entsteht, erfassen Krebsrisikofaktoren und suchen nach neuen Strategien, die verhindern, dass Menschen an Krebs erkranken. Wir entwickeln neue Methoden, mit denen Tumore präziser diagnostiziert und Krebspatient:innen erfolgreicher behandelt werden können. Jeder Beitrag zählt – ob in der Forschung, in der Administration oder der Infrastruktur. Das macht unsere tägliche Arbeit so bedeutungsvoll und spannend.
The Department Pediatric Immuno-Oncology is looking for as soon as possible a
We are looking for a highly motivated technician to join our growing Junior Research Group of Pediatric Neuro-Immuno-Oncology led by Franziska Blaeschke. Our research group uses advanced genetic engineering to improve immunotherapy of solid tumors. While treatment with chimeric antigen receptor (CAR) T cells has shown great success in pediatric leukemia, CAR T cells are still inefficient in most solid tumors. We have recently pioneered the use of CRISPR-based modular pooled knockin screens to study which (synthetic or natural) gene products can be overexpressed in primary human T cells to enhance the efficacy and fitness of CARs (Blaeschke et al., Cell, 2023).
Ihre Aufgaben:
The technician will play a key role in conducting and advancing in vivo studies, utilizing cutting-edge gene editing technologies (e.g., CRISPR knockin, knockout, CRISPRa, base editing, prime editing) to investigate CAR T cell interactions with tumor cells and enhance their efficacy in solid tumors. The position will primarily focus on in vivo experiments using different mouse models, while also integrating in vitro methodologies. Additionally, the technician will contribute to maintaining the lab’s infrastructure (inventory, ordering, etc.) and closely collaborate with postdoctoral researchers and students.
Your responsibilities:
- Performing in vivo studies using xenograft and syngeneic mouse models, including tumor implantation, CAR T cell injections, tissue collection, and downstream analysis
- Conducting in vitro and ex vivo assays related to immunotherapy, such as cell culture, functional assays (e.g., cytokine release, proliferation, cytotoxicity), and flow cytometry
- Supporting genetic engineering approaches, including cloning, CRISPR/Cas9 modifications, and viral transduction
- Developing and optimizing experimental protocols for in vivo studies
- Ensuring accurate documentation and compliance with animal welfare regulations
- Assisting in maintaining the lab’s infrastructure (e.g., inventory, ordering, organization)
- Collaborating with postdoctoral researchers, students, and other technicians.
Ihr Profil:
- Qualification as BTA (Biological Technical Assistant), MTA (Medical Technical Assistant), or biological laboratory technician (Biologielaborant:in) (m/f/d)
- Excellent communication skills and social competence
- Team player who is motivated to closely work with postdocs, students and other technicians within the lab and beyond
- Willingness to perform in vivo studies
- Strong experience in handling laboratory animals and conducting in vivo procedures is an advantage
- Previous experience in cloning, CRISPR/Cas gene engineering, viral transduction, CAR T cells and primary cell culture is helpful but not mandatory
- Proficiency in English and German (written and spoken)
- High level of enthusiasm, motivation and responsibility
- Effective time management and organizational skills
Please submit a motivation letter (1 page), a CV and two letters of recommendation in one single pdf.
If you would like to apply part-time, please state in your letter of motivation the percentage of time you will be working part-time.
Unser Angebot:
- Hervorragende Rahmenbedingungen: modernste state-of-the-art Infrastruktur und Möglichkeit zum internationalen Austausch auf Spitzenniveau
- 30 Tage Urlaub
- Flexible Arbeitszeiten
- Vergütung nach TV-L inkl. betrieblicher Altersvorsorge und vermögenswirksamer Leistungen
- Möglichkeit zur Teilzeitarbeit
- Familienfreundliches Arbeitsumfeld
- Nachhaltig zur Arbeit: Vergünstigtes Deutschland-Jobticket
- Entfalten Sie Ihr volles Potenzial: gezielte Angebote für Ihre persönliche Entwicklung fördern Ihre Talente
- Unser betriebliches Gesundheitsmanagement bietet ein ganzheitliches Angebot für Ihr Wohlbefinden
Sie sind interessiert?
Dr. Franziska Blaeschke
Telefon: +49 (0)6221/42-2965
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