Position: Postdoc in Single Cell Mutagenesis
Department: Artificial Intelligence in Oncology
Code number: 2021-0034
The German Cancer Research Center is the largest biomedical research institution in Germany. With more than 3,000 employees, we operate an extensive scientific program in the field of cancer research.
The laboratories of Dr. Moritz Gerstung (Division of Artificial Intelligence in Oncology ) and Dr. Duncan Odom (Division of Regulatory Genomics and Cancer Evolution) study how the cancer genome evolution is shaped by the interplay of DNA damage and repair. To understand how genetic sequences become corrupted and how this can lead to human tumours, our collaborative efforts design, perform, and computationally integrate a diversity of experiments, including single-cell sequencing, functional cell and genome analysis, and spatial transcriptomics. The Gerstung lab will join the Functional and Structural Genomics programme of the German Cancer Research Center (DKFZ), which is home to the Odom lab, in the dynamic and exciting academic landscape of Heidelberg in the summer of 2021.
This collaborative project will dissect how chemotherapeutics and related mutagens interact with DNA repair pathways by a systematic and single-cell based assessment of exposure experiments coupled to microfluidics, live cell imaging, whole genome and transcriptome sequencing, and cellular phenotyping. Cells from different DNA repair deficient human cell lines will be exposed to genotoxins at different doses and cultivated for 5-10 generations to create single-cell derived colonies. The underlying distribution of DNA lesions, their genotoxic triggers, estimated repair efficiency from different knockouts and segregation rates, as well as the kinetics of pro- and antimutagenic forces from each component of DNA repair will be quantified. One possible direction is to further study the evolution of complex structural variants arising from stalled replication, which cannot be overcome by translesion synthesis, particularly in TLS deficient lines.
The postdoctoral fellow will be responsible for project management, experimental method development and optimisation, collection and analysis of novel and large genomic datasets. In addition, close collaboration with computational biologists, as well as direct training in advanced bioinformatics methodologies, will be expected
We are seeking a postdoctoral research fellow with extensive molecular biology/genomics expertise to join our efforts who is collaborative, ambitious, meticulous, scientifically adventurous, and curiosity-driven. Prior research experience in single-cell methodologies, spatial transcriptomics, transcriptional regulation, epigenetics, and bioinformatics would be advantageous; we also encourage applications from accomplished graduate students seeking to switch research fields.
- Interesting, versatile workplace
- International, attractive working environment
- Campus with modern state-of-the-art infrastructure
- Salary according to TV-L including social benefits
- Possibility to work part-time
- Flexible working hours
- Comprehensive further training program
- Access to the DKFZ International Postdoc Program
Earliest possible start date: as soon as possible
Duration: The position is initially limited to 2 years with the possibility of prolongation.
The position can in principle be part-time.
Application deadline: 06.03.2021
Phone +49 (0)6221/42-2747
Please note that we do not accept applications submitted via email.