Position: Postdoctoral Scientist in Spatial Genomics
Department: Artificial Intelligence in Oncoclogy
Code number: 2021-0362
The German Cancer Research Center is the largest biomedical research institution in Germany. With more than 3,000 employees, we operate an extensive scientific program in the field of cancer research.
The laboratory of Dr. Moritz Gerstung (Division of Artificial Intelligence in Oncoclogy) at the German Cancer Research Center is looking for a postdoctoral fellow in spatial genomics. The group studies cancer evolution using a combination of AI analysis and new experimental technologies [1,2]. We have recently co-developed experimental protocols to detect mutations in tissue sections which map how cancers have grown in their tissue context . This method, termed base specific in situ sequencing (BaSISS) extends the Cartana/10x ISS protocol. The BaSISS protocol combines sequencing of a tumour to identify mutations and localises these variants in a tumour section with single molecule padlock probes, which are interrogated by cyclic fluorescence microscopy. This generates signals from 100,000s of cells in a tissue, which are assembled into maps of subclonal growth. The protocols also allow for measuring the histopathological and transcriptomic changes acquired during tumour progression.
We are looking for an experimental postdoc to implement and run these spatial genomics and transcriptomics technologies in our new lab at DKFZ which opened in September 2021.
- Fu, Y. et al. Pan-cancer computational histopathology reveals mutations, tumor composition and prognosis. Nature Cancer 1, 800–810 (2020)
- Gerstung, M. et al. The evolutionary history of 2,658 cancers. Nature 578, 122–128 (2020)
- Lomakin, A. et al. Spatial genomics maps the structure, character and evolution of cancer clones. bioRxiv (2021) doi:10.1101/2021.04.16.439912
- Implement and run BaSISS and general ISS experiments
- Run 10x visium spatial transcriptomics arrays on fresh and fixed tissues
- Implement other standard in situ hybridisation assays (e.g. RNAscope, Nanostring GeoMx) widely utilized in the field
- A PhD in biological sciences or related field, with a focus on histology
- Advanced experience with tissue preservation (e.g. fixation, cryo-preservation) and sectioning including both paraffin embedded and frozen tissue material
- Experience in fluorescence microscopy of cells or tissues
- A proven track record of delivering ambitious biomedical research projects
- Excellent communication skills and ability to work effectively within a team
- Experience with immunohistochemistry and in situ hybridization
- Knowledge of image data pre-processing
- Ability to test, implement and troubleshoot new techniques and protocols
- Attention to detail and record keeping for sample and experimental tracking
- Interesting, versatile workplace
- International, attractive working environment
- Campus with modern state-of-the-art infrastructure
- Salary according to TV-L including social benefits
- Possibility to work part-time
- Flexible working hours
- Comprehensive further training program
- Access to the DKFZ International Postdoc Program
Earliest possible start date: as soon as possible
Duration: The position is initially limited to 2 years with the possibility of prolongation.
The position can in principle be part-time.
Application deadline: 07.12.2021
Dr. Moritz Gerstung
Phone +49 (0)6221/42-4522
Please note that we do not accept applications submitted via email.